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CM-Dil+ and CM-DiI-negative populations were isolated using fluorescence-activated cell sorting (FACS) at indicated timepoints

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EVs isolated from 1 mL of the supernatant were resuspended in 100 L of PBS

2.5 Ligand preparation for docking Three FDA-approved drugs from the SSRI family (Sertraline, Fluoxetine, and Citalopram) and two known chemotherapeutic drugs (Doxorubicin and Vincristine) were selected for molecular docking

Researchers who can tolerate that hunger window and wait before eating will see significantly better GH response than those who eat immediately

however, neither dataset showed detectable expression of Mtnr1a or Mtnr1b in these cells ( Supplementary Figure 3C )