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Materials and Methods Animals High-fat diet (67% maintenance diet for rats and mice +10% lard +20% sucrose +2.5% cholesterol +0.5% sodium cholate) feedstuff and 3-week-female C57BL/6J mice were purchased from Pengyue Experimental Animal Breeding Co Ltd

Step 4: Monitor protein intake

Under oxidative stress and induced by excessive ROS generation, Nrf2 is released from its inhibitor Keap1, allowing its translocation into the nucleus (Kasprzak et al., 2020), where it binds to the antioxidant response element (ARE) present in the DNA sequence of numerous antioxidant enzymes like glutathione-S-transferase, -glutamyl cysteine synthetase (glutamate cysteine ligase), heme oxygenase 1, and paraoxonase-1 inducing their transcription, managing the phase II response to oxidative stress (Kasprzak et al., 2020)

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