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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR
Last reviewed: Jan 2020.
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Unlike many peptides, BPC-157 is remarkably stable in human gastric juice for extended periods (over 24 hours), a property that is unusual for a peptide of its size and that makes it viable for both injectable and oral research protocols
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