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[13] The first reaction of the carnitine shuttle is a two-step process catalyzed by a family of isozymes of acyl-CoA synthetase that are found in the outer mitochondrial membrane , where they promote the activation of fatty acids by forming a thioester bond between the fatty acid carboxyl group and the thiol group of coenzyme A to yield a fatty acylCoA

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Compared with baseline, the results showed a significant increase in collagen fiber network (fiber cross-linking and fiber length) along with a significant increase in epidermal hyaluronic acid levels