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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)
This is the first threshold of enhancement, he said
Attributing long-term changes directly to the sustained presence of the peptide would be a misinterpretation of its fundamental kinetics
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