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Cell were then treated with Cy5.5-labeled NP4 for 1 h, 4 h, or 7 h, respectively, washed with PBS, and analyzed by flow cytometry (Beckman Coulter, U.S.A.)

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(MOBILE NUMBER on water mark is NOT ACTIVE) so far ok na ok sakin ang TAD same sila ng effect ni germed sakin mas ma glow lang ang TAD at least naka hanap nadin ako ng kapalit ng germed if incase oos na naman

The LDH assay was performed to validate the results obtained from the MTT assay

Band intensities were quantified by densitometry

IGF-1's suppression of GH secretion through negative feedback indirectly improves metabolic function by reducing GH-mediated lipolysis and its associated insulin-antagonistic effects