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Mechanism-based inhibition of iPLA2 demonstrates a highly reactive cysteine residue (C651) that interacts with the active site: mass spectrometric elucidation of the mechanisms underlying inhibition
As long as you don't have a copper metabolism disorder like Wilson's disease, copper toxicity isn't a concern
The five most upregulated genes in microglia/macrophages were HSPH1, HSPB1, HSP90AA1, CHORDC1 and PTGES3, while the five most downregulated genes were CD14, SLC2A3, CCDC26, HAMP and TMEM163 (Fig
If the protein was in one of the non-pellet fractions, your protein got expressed and was soluble, so the problems something with your purification
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