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During the entire experiment, the mice were housed under rigorously controlled conditions, including a temperature of 23C 2C, a relative humidity of 45% 5%, and a consistent 12-hour light/dark cycle

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For instance, the expression level miRNA-422a is negatively correlated with metastasis of hepatocellular carcinoma (HCC), and miRNA-422a is significantly downregulated in HCC (33)

Statistical significance in e was determined by one-way ANOVA followed by Bonferroni post-hoc analysis

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