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Beyond these examples, exercise also activates broader metabolic, oxidative stress, immune, and mechanotransduction programs, such as AMPK-PGC1 signaling [14,15,16], Nrf2-ARE antioxidant signaling [17], JAK-STAT immune signaling [18, 19], and mechanosensitive modules, including integrin-FAK-RhoA/ROCK signaling [20, 21], YAP/TAZ-Hippo signaling [22,23,24], and Piezo1-calcium signaling [25]

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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

PLoS ONE, 7 , e40433

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