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The cell suspensions were washed twice with ice-cold PBS before staining using an Annexin V/PI staining kit (Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit)

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Your next step If you want to try GHK-Cu at home, start with GHK-Cu and keep the rest of your routine calm for two weeks so your skin can tell you how it reacts

a.k.a UMN in mice) of well characterized mouse models of TDP-43 pathology, both display prominent mitochondrial defects in a cell-type specific manner 16

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