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Major sites of SULT2A1 expression are the liver, adrenal gland, ovary, and duodenum (Javitt et al., 2001)

Proteins were fractionated electrophoretically on NuPAGETM 412% Bis-Tris Polyacrylamide Gel (Invitrogen) and then transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot Dry Blotting System (Invitrogen)

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Significance is indicated with asterisks * and a minimum P value of 0.05 was considered statistically significant (refer to figure legends for details)

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Also, people with diabetes who take a medication called metformin are at a higher risk of developing B12 deficiency because this medication can reduce B12 absorption