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Pregnant or breastfeeding people should avoid use due to lack of safety data in these populations
For tumour xenograft experiments, athymic nude NU/J mice (The Jackson Laboratory, 002019) were used (female, at least 8 weeks old)
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Mitochondria were treated with pyruvate/malate/FCCP followed sequentially with rotenone, succinate, antimycin A, and N,N,N,N-Tetramethyl-p-phenylenediamine (TMPD)/ascorbic acid (n = 5)
doi:10.4103/2321-4848.123023
The combination of convenient dosing intervals and sustained drug presence makes weekly administration both practical and effective for ongoing treatment
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