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My hands and feet, which used to get cold easily during Canadian winters, seemed warmer

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When oxalate levels are out of balance, problems can arise with oxalate crystal formation, which can cause kidney stones, joint pain, or other problems

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So, to these, what I normally do is: 1) add 50uL 1X SDS loading buffer (this has a detergent so it will help small-scale lyse things but it will also solubilize things so you wont be able to tell if your protein was soluble) 2) boil 10-15min 3) centrifuge briefly 4) load supernatant (~5uL) Now, as promised, SUMOre on SUMO

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