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Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)

The concentration of TNF- and IL-6 in the supernatant and serum harvested above was measured by using enzyme-linked immunosorbent assay kits (Dakewe, Beijing, China) according to the manufacturer's instructions