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Regular intake helps maintain steady glutathione production over time
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However, in glutathione synthetase deficiency, because the activity of gamma-glutamyl-cysteine synthetase is regulated via feedback inhibition by glutathione, gamma-glutamyl-cysteine and then L-5-oxoproline are overproduced, resulting in the metabolic acidosis and urinary excretion of this compound at levels several hundred-fold above normal (71
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2006 ) , a G1/S inducer, whose expression was also negatively affected by MTX (Fig