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It is known that peptide 20 potently reversed metabolic disorders in rodent models of obesity and diabetes, characteristic of increased energy expenditure and elevated circulating FGF21 levels as a result of GCGR agonism 22,23

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The absorbance of the resulting precipitate was measured at 425 nm using a microplate reader (Infinite E Plex, Tecan, Mnnedorf, Switzerland)

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