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6.3.1 ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) methodology The ABTS protocol evaluates antioxidant efficacy through the neutralization of pre-generated ABTS + radical cations ( + through either single electron transfer (SET) or hydrogen atom transfer (HAT) pathways, leading to radical quenching and a proportional reduction in measured absorbance ( 6.3.2 FRAP (ferric reducing antioxidant power) methodology FRAP analysis determines sample reducing capacity by measuring electron donation potential ( 3+ -TPTZ) complexes to their ferrous counterparts (Fe 2+ -TPTZ)
For some patients, tiredness is not caused by the medication alone
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Proposed pathways include modulation of multiple neurotransmitter systems (influencing serotonin, dopamine, norepinephrine, GABA, glutamate levels), possible involvement of NMDA receptors, alpha-1 adrenergic signaling (e.g., potentiating pineal acetyltransferase activity), inhibition of somatostatin release via dopaminergic routes, reduced ACTH/cortisol for stress buffering, and stimulated growth hormone or somatoliberin secretion
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