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The div-1(or148) mutants displayed embryonic lethal phenotype due to delayed embryonic cell division at 25C (Encalada et al., 2000)

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Following immunostaining, L3 central nervous system (CNS) or body walls were mounted in SlowFade Gold (Invitorgen) and fluorescent images were acquired on a LSM 980 (Carl Zeiss) using a 20 objective (for the CNS) or 40/63 objective (for the NMJ)

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