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Single-nucleus RNA data preprocessing, clustering and annotation The raw gene expression matrix was filtered after removal of cells with either more than five mean absolute deviations more mitochondrial gene expression unique molecular identified counts, fewer than 500 detected genes or with more than 5 mean absolute deviations in total unique molecular identified counts

For posterior sites, bend the knee to 30 degrees

( C ) RNA-seq values of group I vs group II (Fig

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