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The relative activities at each pH value (expressed as percentages) were normalized to the maximum activity observed for each variant in the assay

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None of these have been formally tested with retatrutide+cagrilintide

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Metabolite measurements using LCMS For aqueous metabolites extraction, serum (5 l) was mixed with 150 l of extraction solvent (40:40:20 methanol:acetonitrile:water, v:v:v) at 20 C, vortexed and immediately centrifuged at 16,000 g for 10 min at 4 C