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Phase 2 NEJM titration (still common in research) Phase 2 retatrutide titration schedule Phase Initiation Weeks Weeks 1-4 Weekly Dose 1 mg Phase Early escalation Weeks Weeks 5-8 Weekly Dose 2 mg Phase Mid escalation Weeks Weeks 9-12 Weekly Dose 4 mg Phase High escalation Weeks Weeks 13-16 Weekly Dose 8 mg Phase Maximum Weeks Weeks 17+ Weekly Dose 12 mg Source: Jastreboff et al., NEJM 2023

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Several ATG-related proteins were identified and their role in the molecular mechanisms of ATG highlighted

Soluble His-tagged GpoA protein was purified using nickel affinity chromatography following manufacturers instructions (Ni-NTA agarose, Qiagen)