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Besides, a phosphate-restricted diet upregulated the expression of Klotho in the kidneys of a mouse model of polycystic kidney disease (115)

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Briefly, 100 l of enzyme conjugate was added to the wells containing 50 l of standard or plasma, covered with an adhesive strip and incubated for 60 min at 37 C

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doi: 10.1530/joe-19-0385 86 NazninFSakodaHOkadaTTsubouchiHWaiseTMZArakawaKet al

With 5 mL BAC water, those draws increase to 10 and 30 units