Cellular ROS levels were assayed to evaluate cell rescue redox states with the cytosolic oxidant-sensitive probe 2,7-dichlorodihydrofluorescein diacetate (DCFHDA), which measures the oxidative conversion of DCFHDA to the highly fluorescent compound dichlorofluorescein (DCF)
"What we are advocating for is not the Wild West," said Brigham Buhler, who runs a telehealth company that offers hormone optimization and peptides and is an ally of Secretary Kennedy
For cells, they were first washed with PBS and fixed at room temperature for 15 min, followed by incubation with the staining solution at pH 6.0 overnight at 37 C
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Follow institutional protocols for reconstitution and aseptic handling
The agency had posted no final list addition for any of the seven peptides at the time of this update