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The cells were treated with 10 mM EtGSH in FluoroBrite DMEM for 34 h, 1 mM BSO in FluoroBrite DMEM for 34 h, 1 mM H 2 O 2 for 20 min in FluoroBrite or vehicle (FluoroBrite DMEM)

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First, Vitamin B12 plays a critical role in cellular energy metabolism, an essential process for testosterone synthesis

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After recovery, you might cautiously retry at lower concentration or frequency

This results in systemic signaling effects that extend beyond adipose tissue, making these compounds more suitable for studying integrated endocrine regulation rather than isolated metabolic pathways