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The direct comparison upon using the same concentration of ruthenium of the molecular agent Ru(II)-OH and NP4 showed a significantly higher tumor accumulation of the nanoparticle formulation NP4 (Supplementary Fig
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The intracellular glutathione levels were measured colorimetrically based on reduction of 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) into TNB by glutathione
Collectively, these investigations suggested the plausibility of modulating the rate of drug release as well as specifically targeting different GSTs by modifying the sulfonamide moiety 128
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