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65,78,79,80,81,82,83,84,85,86 Thus, at present, non-proliferative and proliferative astrocyte reactivity represent two broad categories that can be readily differentiated and are associated with diverse molecular and functional differences

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2022;119(30):e2119368119

Benzoyl chloride (0.57 mL, 4.93 mmol) was slowly added and the mixture stirred overnight while warming to ambient temperature

The major member of class II NPFs is cortactin

The protein was transferred to 0.2 m pore-sized nitrocellulose (wet transfer, 1 hour, 100 mA, RT) and then blocked with 5% non-fat dry milk in TBS-T (20 mmol/L TrisHCl, 150 mmol/L NaCl, 0.05% Tween 20, adjusted to pH 7.4) and incubated with the following primary monoclonal antibodies: anti--actin, anti-cleaved-PARP, anti-cleaved Caspase-3, anti-cleaved Caspase-9, and anti-COX IV (for apoptosis evaluation) or anti-LC3A/B, anti-Beclin-1, anti-Atg12, and anti-Atg-7 (for autophagy evaluation) antibodies, diluted 1:1,000 in blocking buffer overnight at 4C