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The bound protein was eluted using 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, and 5 mM EGTA and 0.1 mg/mL Flag peptide

Several studies using AstaPure astaxanthin have shown improvements in reduced water loss and increased skin elasticity (44 Healthy Female Adults)[iii] on wrinkle parameters and skin moisture analysis in (39 healthy female adults)[iv]( 16 healthy female adults)[v] Fine Line Reduction Smoother, Flawless Skin Both oral and topical use of astaxanthin has shown to help reduce the appearance of fine lines and wrinkles

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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C