In vitro experiments demonstrated that agrin treatment significantly downregulated PDGFR and claudin-5 expression in a blood-brain barrier co-culture model comprising human brain microvascular endothelial cells and pericytes, suggesting that agrin may induce pericyte death and impair the integrity of the blood-brain barrier
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Minimize air exposure by: Using appropriate reconstitution volumes (don't leave excessive headspace) Limiting the number of needle punctures Purging vials with inert gas (nitrogen or argon) if available How long do peptide solutions actually last
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