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Sigma, 4693159001) on ice for 10 min, then sonicated using a Diagenode BioRuptor Pico (Diagenode, B01060010) for ten cycles (30 s on, 30 s off) at 4 C

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3 Result 3.1 Annual publications The complete retrieval and analytic process was shown in Figure 1

These methods reduce waste and create stable solutions that store well