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A 10 IU vial in 1 mL of BAC water yields a 10 IU/mL concentration, so 1 IU equals 0.1 mL or 10 units on a U-100 insulin syringe

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The fragmented RNA was then divided into two conditions: m 6 A-RIP, incubated with 1 g of m 6 A-specific antibody, and IgG control, incubated with 1 g of IgG, rotating head over tail for 20 h with gentle rotation at 4 C