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10.1146/annurev-neuro-061010-113810 48 StephanA

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You enter: Peptide mass in the vial (mg) Bacteriostatic water volume added (mL) Research dose per unit (mcg or mg) Syringe type (typically 100-unit / 1mL) The calculator returns: Concentration (mg/mL and mcg/mL) Peptide per syringe unit (mcg/unit) Units required for the specified research dose Total research doses per vial This saves time and eliminates arithmetic errors, especially for multi-vial research protocols or when switching between peptides with different vial sizes

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Steaming for 2-3 minutes is optimal