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We chose (EAAAK) 3 rigid helical linkers to work because we believe their rigidity allows for the separation of the protein domains and improves the functionality of individual domains 58,59 when compared to flexible linkers, which appear to be suitable for linking normally connected domains in a single genetic construct, such as antibody domains in scFvs 60

This work was financially supported in part by National Institutes of Health awards U01HG011715, R01HG009120 and R01MH115676

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