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doi: 10.1167/iovs.17-22467
(The supernatant can be stored at -80 for 10 days.) Cell sample Harvesting cell should not less than 106,then wash with PBS for twice (mix cell with PBS centrifuge at 600g for 10 minutes), mix precipitated cell with the volume of PBS for 3 times
In addition, it has long been known that GSH is capable of forming disulfide bonds with cysteine residues of proteins, and the relevance of this mechanism (S-glutathionylation) in regulation of protein function has been well documented in a number of research fields
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The role of diet, additives, and antibiotics in metabolic endotoxemia and chronic diseases
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