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Growth hormone therapy

After diluting with 50 mM ammonium bicarbonate so that the final concentration of SDC and SLS was 2.4 mM, extracted proteins were digested with 1 g of MS grade trypsin (Thermo Fisher Scientific) for 16 h at 37 C

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While the vial is lying on the table, insert the clean needle into the top of it and inject the air into the vial