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For the fed-batch culture in the 5-l bioreactor, medium containing 15 g/L Na 2 HPO 4 12H 2 O, 3 g/L KH 2 PO 4 , 0.5 g/L NaCl, 3 g/L NH 4 Cl, 0.2 g/L MgSO 4 7H 2 O, 0.011 g/L CaCl 2 , 0.5 mL/L vitamin B1 (1 % w/v), 0.5 mL/L of a stock solution of trace elements, and 4 g/L glucose was used

Maquart FX, Bellon G, Chaqour B, et al

doi: 10.1186/s41936-020-00190-y 110 HeS.WangQ.LiS.RanC.GuoX.ZhangZ.et al

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These results approved that with increase in time of reaction at a moderate temperature, mean diameters and standard deviations of the Ag-NPs gradually increases