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In the co-culture system of VECs and VSMCs under high glucose and high phosphorus microenvironment, VECs secrete a large amount of lactate, which directly leads to the transformation of VSMC osteogenesis
Cells were subsequently cultured in RPMI/10% FBS with LPS/IL-4 for 3 days, harvested and acquired on a LSR Fortessa flow cytometer
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It has been shown that lower densities prime cells for hemolysis and eryptosis [398]
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