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Furthermore, both GIP 100 nM and Tirzepatide at 10 nM stimulated lipolysis through cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, as evidenced by increased glycerol release and HSL phosphorylation on day 19 differentiated cells

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The substantial increase in plasma bile acid concentrations (primary, secondary, total and unconjugated bile acid concentrations) with metformin may be explained by the fact that metformin increased the abundance of probiotic bacteria, especially Lactobacillus , and thus their bile salt hydrolase (BSH) gene expression

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