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RNA was isolated from brain and islets using RNeasy micro kit (Qiagen), cDNA was synthesized from equal amounts of RNA using SuperScript III First-Strand Synthesis kit (Invitrogen) and gene expression was quantified using PowerUp Sybr Green Reagents (Invitrogen) according to the manufacturers instructions
Acta Physiologica
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34 , 5974.e10 (2022)
Vigo MB, Perez MJ, De Fino F, et al
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