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In the Sinha et al
Factors such as tissue source, donor age, in vitro passage number, culture conditions (including hypoxic versus normoxic preconditioning), inflammatory priming, cryopreservation methods, and the lack of standardized potency assays can markedly influence both the intrinsic metabolic profile of MSCs and the composition of their therapeutic secretome, resulting in variable therapeutic efficacy [19,20,21,22,23,24]
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lixisenatide), LEADER (Liraglutide Effects and Action in Diabetes: Evaluation of cardiovascular outcome Results
The dilution of standards, addition of samples, warming, liquid preparation, washing, addition of enzyme, and then addition of color developer were performed sequentially according to the instructions of the kit, and finally, the reaction was terminated to determine the optical density (OD) value and to calculate the actual concentration of the samples
