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The pellet was resuspended in 1% digitonin buffer, incubated for 10 min on ice, and centrifuged at 10,000 g for 5 min at 4 C
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It was found that ALPPS surgery caused an increase in IHH secreted by activated HSCs, and activated the HH signaling pathway in hepatocytes to promote the accumulation of GLI1 in the nucleus, thereby promoting LR (Langiewicz et al., 2017
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The injury pattern, not personal preference, determines which one makes clinical sense
