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However, mitochondrial dysfunction can lead to a buildup of ROS due to an imbalance in their production and degradation 4,5 Another major source of ROS is NADPH oxidase (Nox), a membrane-bound enzyme complex that produces superoxide by transferring an electron to oxygen from NADPH

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To the tissue lysate, ATP monitoring enzyme was added and the luminescent reaction was immediately analyzed in a microplate reader (Spectra Max MII, Molecular Devices, Germany)