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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

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43 In addition to biomechanical factors, the molecular biophysics of the ACL plays a crucial role in understanding injury mechanisms and developing effective prevention and treatment strategies