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Whole-cell biocatalyst The GshF-overexpressing cells were obtained by centrifugation of the induced culture at 12,000 rpm and 4 C for 5 min, followed by two washes with 50 mM phosphate buffer (pH 7.0)

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At standard dosing of 1-2mg daily, a 100mg vial provides roughly 50-100 days of supply

Appl Microbiol Biotech 62:239243 Eiteman MA, Altman E (2006) Overcoming acetate in Escherichia coli recombinant protein fermentations

Theoretical concerns Copper accumulation: Each 2mg injection contributes about 0.03mg copper versus 0.9-2.2mg from daily diet