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To further assess the severity of iron deficiency, additional markers like transferrin saturation (TSAT, the percentage of the plasma protein transferrin that is bound with iron, which is decreased with deficiency) may be used
However, while the final outcome of mitochondrial dysfunction is cell death, the phenotype of death (apoptosis and/or necrosis) depends on the level of cellular ATP, as ATP is required for the efficient assembly of the apoptosome
Because of its defined molecular profile, the compound is frequently used in laboratory studies examining metabolic regulation and biochemical processes
Using the solutions while they are still cold may interfere with the reconstitution process
Moreover, H 2 O 2 , one of the most studied reactive oxygen species in plants, did not induce any detectable reduction of PMA at the tested concentrations, reinforcing the selectivity of the method toward biological reductants rather than general oxidants