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TXNIP downregulates V-maf musculoaponeurotic fibrosarcoma oncogene homolog A (MafA), a transcription factor to drive insulin expression, and thus negatively regulates insulin expression 25

Following the removal of the His-SUMO tag by ULP1, the eluted protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 GL column with an elution buffer containing 20 mM HEPES (pH 7.4) and 200 mM NaCl

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Purification was performed by silica gel chromatography, with a yield of 67%

doi:10.1038/nm1366