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May 2005;18(2):120-127
Cells were transduced with lentiviruses in the presence of 10g/mL polybrene (Sigma, 107689), selected and maintained in Complete media containing 2g/mL puromycin (Bioshop, PUR333) for 2-3 days
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There has been noted decrease in Adrenocorticotropic Hormone sensitivity of Adrenal cells expression of negative feedback mechanisms
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