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Discussion The original method faced several challenges, including the need for strong acids to terminate the enzymatic reaction, which could be hazardous and affect the accuracy of the measurement

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This proposal was based in part on the finding that [GSH]:[GSSG] in the secretory pathway and in microsomal vesicles is similar to the ratio found in redox buffers that are effective for the oxidative folding of proteins in vitro that is, between 1:1 and 3:1 (Bass et al , 2004