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Technical duplicates were obtained by separating supernatant samples into two wells of primary antibody-conjugated plates
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Fertil Steril 95(3):10731079 Enechukwu CI, Onuegbu AJ, Olisekodiaka MJ, Eleje GU, Ikechebelu JI, Ugboaja JO, Amah UK, Okwara JE, Igwegbe AO (2019) Oxidative stress markers and lipid profiles of patients with polycystic ovary syndrome in a Nigerian tertiary hospital
confirming that each peak corresponds to the expected component requires mass spectrometry (LC-MS) identification in addition to HPLC purity percentage