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The prevalence of diabetes in Western Europe is rising, fueling the demand for insulin and GLP-1 receptor agonists

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191 Variants (A allele to G allele) within IGF-1 (insulin-like growth factor 1) contribute to its low plasma levels, and cause a reduction in insulin sensitivity

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The resultant cassette named KTP was cut with Eco RI and Hind III and isolated by agarose gel electrophoresis